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    Testing Small Dataset         

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In order to test MetaTrans tool, you can run this small dataset
composed of six samples that were extracted in two time points,
before and after a flatulogenic diet. Read our paper for more details.

The database to be used is a reduced version of MetaHIT 2014 database,
which means that poor results are expected after the analysis. In turn,
the user will experience a faster downloading and data processing.

Taxonomical and functional analysis using "M5nr" are not going to
work with this small test if you try to use them.

To run this test, you should do the following steps:


1) Download the following files:

          Executable files  (.tar.gz) [1-Scripts.tar.gz]
          Third party tools (.tar.gz) [2-ThirdpartyTools.tar.gz]
          Samples-Test (.tar.gz)      [Samples-Test.tar.gz]
          Databases-Test (.tar.gz)    [Databases-Test.tar.gz]

and uncompress them following this order in a working folder. Make
sure you first uncompress: 1-Scripts.tar.gz, 2-ThirdpartyTools.tar.gz.
Overwrite and merge any file/folder you are prompted for :

          tar xvf 1-Scripts.tar.gz
          tar xvf 2-ThirdpartyTools.tar.gz
          tar xvf Databases-Test.tar.gz
          tar xvf Samples-Test.tar.gz

2) Configure the pipeline by running this command in the working folder :

          ./config

3) Edit settings.txt file and setup this two variables to fit your available resources
if these defaults are not suitable:

          THREADS=20              (select number of threads to use in the pipeline)
          SORTMERNA_MEMORY=5      (use MAX to set maximum memory allowed: 11.7GB RAM)

4) Run the pipeline :

          ./metatrans